Plate Runner Platform

Left: photo of the Plate Runner HD from Trophos. Upper right: whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells grown on mouse feeders, stained for pluripotent markers NANOG (red) and OCT4 (green). The cell nuclei is stained with DAPI (blue). Lower right: whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells-derived Hb9::GFP motor neurons stained using calcein.

HCS/HCA enables automated acquisition, visualization and quantification of a large number of images.

The Plate Runner HD allows: 

• Rapid snapshot acquisition of fluorescence images of cells and small organisms
• High resolution full well / full plate imager 
• High through-put format for 96/384 plates
• May serve as a sensitive plate reader detecting whole well fluorescence 

Examples of fluorescence assays that may be measured: 

• Reporter gene assays 
• Cell viability, cytotoxicity and apoptosis assays
• Neurite outgrowth 
• Screening of fast kinetics

This device is very simple to learn and to use. It produces only full standard tiff images, compatible with any vendor image analysis program. 
The Plate Runner is available for everyone to use. 

Services

Assistance with:

• Setting up image acquisition
• Setting up analysis protocols
• Assay validation
• Trouble shooting
• Participating in developing new protocols and techniques

Training:
One-to-one hands on training for image acquisition
Introduction to Plate Runner instrument takes 1 hour.

Planning:
Contact anna [dot] hammarberg [at] med [dot] lu [dot] se and book a time for consultation and planning 

Execution:

• All sample preparation should be done by the user group. 
• Consumables for sample preparation are not included in the user fee. 
• Anna may do data acquisition in smaller projects and pilot experiments. For repeated experiments the user is gradually trained to become independent. Anna will be available for instructions and trouble shooting.

Booking

• All users that require assistance book by e-mail to anna [dot] hammarberg [at] med [dot] lu [dot] se
• First-come, first-served policy will be applied
• Independent users book the Plate Runner in the calendar in the instrument room D1023a.

Do not hesitate to book time well in advance, or ask for last minute service if needed. 

Charges

MultiPark User fee:
50 SEK/hour

External User fee:
150 SEK/hour

Introduction fee: 
500 SEK /hour
Introduction to Plate Runner instrument takes 1 hour.

Technical information

Technical Information Plate runner

Papers published using this system

https://www.nature.com/articles/s41598-019-46110-1
https://www.pnas.org/content/116/52/27053.long
https://www.biorxiv.org/content/10.1101/335372v1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5900565/