Simoa Analyzer

The Simoa Analyzer is particularly useful for measurements of biomarkers at ultra-low levels.

The Simoa Analyzer system features:

• Single molecule array technique for high sensitivity and broad dynamic range
• Combined digital and analog immunoassay dynamic range is >4 logs
• A range of sample types include serum, plasma, cerebrospinal fluid, urine, cell extracts
• Fexibility to design assays to detect both protein and nucleic acid biomarkers
• Detection of nucleic acid levels, including miRNA, without PCR
• Single and multiplex assays
• Multiplexing up to six analytes
• Semi-automated assay preparation using standardized benchtop devices
• Simple 2-step or 3-step workflow with minimal user intervention
• Low volume requirements
• Wide menu of available kits
• Full line of components for user-developed assays

To learn more, visit the official Quanterix SR-X website.

The Simoa is available for everyone to use.

Assay Kits

A menu of Simoa assay kits (single plex and multiplex) are available to measure critical biomarkers. It is also possible to request a custom-designed assay.
Examples of multiplexing Simoa Assay Kits for SR-X:

• Neurology 3-Plex A (Tau, Aß42, Aß40)
• Neurology 4-Plex A (Tau, NF-Light, GFAP, UCH-L1)
• Cytokine 3-Plex A NP (IL-10, IL-6, TNFα)

A full list of assay kits available for the SR-X can be found here on the Quanterix website.

Booking

• All users that require assistance with how to operate the instruments, please contact pilar [dot] lorenzo [at] med [dot] lu [dot] se (pilar[dot]lorenzo[at]med[dot]lu[dot]se)
• Please note that you book the Simoa microplate shaker/washer and the SR-X reader separately in the booking calendars at respective instrument.

Since there are two shaker/washer systems, it is absolutely required that you book the SR-X before running your samples.

• Independent users may book the Simoa shaker/washer in the calendar in the instrument room C1113a
• To get access to room C1113a, please contact pilar [dot] lorenzo [at] med [dot] lu [dot] se
• Independent users may book the Simoa SR-X in the calendar in the instrument room B1113

• To get access to room B1113, please contact anna [dot] hammarberg [at] med [dot] lu [dot] se (anna[dot]hammarberg[at]med[dot]lu[dot]se)

The instrument is available to independent users all days.

Charges

The following charges apply:

MultiPark users: 100 SEK/plate
Non-MultiPark users: 200 SEK/plate

Technical Information

The approach is based on the counting of thousands of single immunocomplexes in femtoliter-sized reaction chambers, which are termed Simoa - Single Molecule Arrays.  Simoa uses the same reagents as conventional ELISA, but antibody capture agents are attached to the surface of paramagnetic beads that are used to concentrate a dilute solution of target molecules. The beads are then loaded to discs containing arrays of femtoliter-sized wells that hold only one bead per well. In wells containing a bead with a single target molecule, the formation of an immunocomplex will result in enzymatic cleavage of a substrate, which will be converted to a fluorescent product imaged by the SR-X reader. 

Additional technical information about Simoa can be found here on the Quanterix website: Principles of Simoa - single molecule array technology.