Two-Photon Microscopy
Two-photon microscopy is the method of choice for imaging living cells and tissues (intravital microscopy). It allows imaging with very high temporal and spatial resolution in thick biological material. This MultiPark platform consists of two microscopy stations that serve different needs. The ‘in vivo’ setup can be used to image the processes in the living mouse, i.e. superficial layers of the cerebral cortex. The ‘ex vivo’ system is designed to image thick live preparations, such as brain regions that are not accessible to ‘in vivo’ microscopy. In this setup, two-photon imaging can also be combined with patch-clamp electrophysiological recordings. Although primarily acquired to perform studies in neuroscience, the system is available to all interested researchers at and outside LU.
Three-step Admission System
- Contact platform responsible (in vivo or ex vivo) and consultation about the envisage experiments and potential use of the platform, incl. on-site visit (free of charge).
- Introduction (mandatory) and supervised pilot experiments, charged as non-assisted use.
- Access to platform and booking system. User will have the possibility to run experiments independent (24/7) or with assistance.
Pricing
Introduction:
700 SEK/h
LU User:
700 SEK/h without assistance
1300 SEK/h with staff assistance
External User:
1400 SEK/h without assistance
2600 SEK/h with staff assistance
Contact
For the “in vivo” microscope:
Karsten Ruscher
Researcher
E-mail:
Karsten [dot] Ruscher [at] med [dot] lu [dot] se
For the “ex vivo” microscope:
Karsten Ruscher
Researcher
E-mail:
Karsten [dot] Ruscher [at] med [dot] lu [dot] se